Effects of D20 and Osmotic Gradients on Potential and Resistance of the Isolated Frog Skin

I N T R O D U C T I O N In 1939 T. C. Barnes (1) reported experiments in which he replaced the inside and outside solutions bathing frog skin with D~O Ringer's (chloride). He observed large decreases in potential. In our initial experiments with D~O effects on frog skin the magnitude of the depression depended upon which side of the skin was exposed to D20: outside exposure gave the largest depression, inside exposure gave the least depression, and simultaneous exposure